Laboratoire d’accueil :
Laboratoire de Nanobiophysique, ESPCI 10 rue Vauquelin 75005 Paris
Sujet de thèse :
Optical tweezers studies of RNA/protein interactions in ribosome assembly
Thématique de recherche :
The present project focuses on the ribosome, the RNA-protein complex (RNP) responsible for protein synthesis in all organisms. The aim is to study ribosome assembly at a single molecule scale. The large subunit of the E.coli ribosome results from the stoichiometric assembly of two rRNA species, and 34 r-proteins. This assembly is highly cooperative in vivo and in vitro. However, the molecular basis for the cooperativity remains largely unknown.