Proposition de sujet de stage en laboratoire Niveau Master – S2

(3 lignes maximum) :
Characterization of phage-host interaction using acoustic waves confinement.

Champ : Biophysique

B) DESCRIPTION DU SUJET (1 page maximum)

1. Présentation et description du sujet

A fundamental issue is to understand how bacteria evolve and adapt to their environment, including challenges posed by antibiotics, phages, nutritional stress. Acoustic traps make possible the containment of a population of bacteria in a volume of 1μl in acoustic levitation while allowing dynamic control of the composition of the ambient liquid medium. Thanks to the volume of the confined population it is possible to directly observe population responses to various stresses by microscopy. Initially, the project aims to optimize the acoustic trap technology to study the model bacterium Pseudomonas fluorescens in an open microfluidics system, and secondly to observe the response of P. fluorescens populations to phage attack using phages isolated by the laboratory of Eaux de Paris, upstream of its water treatment processes.
The internship of 6 months will take place in the international environment of the Laboratoire Génétique de l’Evolution in the Chimie Biologie Innovation Institute of the ESPCI.

2. Techniques/méthodes utilisées

Microfluidics, microscopy, microbiology, acoustic, image analysis.

3. Résultats attendus

Characterization of the interactions of environmental phages on Pseudonomas fluorescens bacteria in acoustic confinement.

4. Références

Gutiérrez-Ramos, S., Hoyos, M., & Ruiz-Suárez, J. C. (2018). Induced clustering of Escherichia coli by acoustic fields. Scientific Reports, 8(1), 1–8. http://doi.org/10.1038/s41598-018-22960-z
Hammarström, B., Laurell, T., & Nilsson, J. (2012). Seed particle-enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems. Lab on a Chip, 12(21), 4296. http://doi.org/10.1039/c2lc40697g
Buckling, A., & Rainey, P. B. (2002). The role of parasites in sympatric and allopatric host diversification. Nature, 420(6915), 496–499. http://doi.org/10.1038/nature01164