ESPCI web site
Laboratoire d’accueil :
In the Laboratoire de Colloïdes et Matériaux Divisés at ESPCI we develop millifluidic tools for the control of droplets dispersed in an oil phase (water/oil emulsion) within tubing. Each droplet is used as an independent microreactor with a volume of 500 nL. Individual operations on droplets are performed in elementary modules. The modules allow droplet production, droplet splitting, incubation, fluorescent detection, and sorting of the droplets. These modules can be integrated to allow complex operations to be performed, including the screening of micro-organisms in droplets. In a single experiment, we can reliably encapsulate 1000 micro-organisms in droplets (single organisms in each droplet) and measure their individual enzymatic activities. This technology allows the selection of the most efficient organisms among a population of variants. For this project we aim to screen organisms for activities permitting the transformation of a range of agro-resources, including the conversion of biomass to bioethanol.
Thématique de recherche :
The candidate will take part in various steps of the development of a millifluidic system dedicated to the screening of micro-organisms, in collaboration with the members of the laboratory already involved in the project :
1. Setting-up a new detection system for biomass measurement and integration on a droplet-based
millifluidic instrument.
2. Integration of multiple modules droplet-processing. (sorting, adding new
compound to the drops).
3. Programing for data aquisition and data processing in real-time : development of a program — on the basis of an exisiting one (Labview NI) — to control all the elements of the millifluidic platform (detections, flow control, electric actuation, image capture...).
4. Final step will be to install a millifuidic system in Soufflet center based on project development
Compétences requises :
The candidate should be a PhD with expertise in one or more of the following fields : optics, hydrodynamics, and engineering. The project is highly multidisciplinary so the candidate should be ready to work and interact with chemists and biologists from both academia and industry.
Additional experience or interest in programming (Labview, Matlab), instrument development, or microbiology would be advantageous but not essential.
Début :
September 1, 2013
Durée :
9 months
Description du sujet :
Industrial partnership between ESPCI (http://www.lcmd.espci.fr) and Soufflet S.A., the leading French agri-food company (http://www.soufflet-group.com/).